EC Number   |
|---|
   2.3.1.299 | DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic tree, recombinant expression of HA-tagged enzyme in HEK-293T cells |
| 2.3.1.299 | expressed in HEK-293 cells |
| 2.3.1.299 | expressed in HEK-293T cells |
| 2.3.1.299 | gene CERS1, ectopic expression of N-terminally FLAG3-tagged CerS1 in HEK-293 or HeLa cells, recombinant expression of enzyme mutant H182A/H183A |
| 2.3.1.299 | gene CERS1, quantitative real-time PCR enzyme expression analysis |
| 2.3.1.299 | gene CERS1, recombinant expression in HEK-293 cells |
| 2.3.1.299 | gene CERS1, recombinant expression in HEK-293T cells, quantitative real-time quantitative PCR enzyme expression analysis |
| 2.3.1.299 | gene CERS1, recombinant overexpression in HEK cells, FTY720 inhibits CerS1 activity to a greater extent than CerS5 in the overexpressing cells |
| 2.3.1.299 | transient recombinant expression of N-terminally FLAG-tagged wild-type and mutant enzymes in HeLa cells, overexpression of wild-type LASS1 increases cellular levels of C18 and C18:1 ceramides by 10fold |
