EC Number |
---|
2.3.1.24 | DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic tree, recombinant expression of HA-tagged enzyme in HEK-293T cells |
2.3.1.24 | DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic tree, recombinant expression of HA-tagged enzyme in HEK-293T cells, and recombinant expression of C-terminally GFP-tagged Lass6 |
2.3.1.24 | expression in Escherichia coli |
2.3.1.24 | expression in HEK-293T cell |
2.3.1.24 | expression of wild-type and mutant enzymes in Escherichia coli strain BJ5183, and transient expression in HEK293A cells, HT29 cells, and SW620 cells from an adenovirus vector |
2.3.1.24 | gene CERS4, isozyme expression analysis |
2.3.1.24 | gene CERS4, quantitative real-time PCR enzyme expression analysis, transient overexpression of HA-tagged CerS4 in INS-1 cells increasing the levels of ceramides C18:0 and C22:0 by 20%, but it has no effect on the accumulation of ceramides C16:0 and C24:0 |
2.3.1.24 | gene CERS4, recombinant overexpression in HEK cells |
2.3.1.24 | gene CERS5, quantitative real-time PCR enzyme expression analysis |
2.3.1.24 | gene CERS5, recombinant expression in HEK-293T cells, quantitative real-time quantitative PCR enzyme expression analysis |