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EC Number Cloned (Commentary) Reference
Display the word mapDisplay the reaction diagram Show all sequences 2.3.1.189cloned in pQE31 plasmid and transformed into Escherichia coli M15 (pREP4) 757534
Display the word mapDisplay the reaction diagram Show all sequences 2.3.1.189expression in Escherichia coli 695916
Display the word mapDisplay the reaction diagram Show all sequences 2.3.1.189the Rv0819 gene encoding MshD (residues 1–315) is amplified from Mycobacterium tuberculosis genomic DNA using PCR and cloned into the pET28a(+) plasmid. This vector construction adds six histidine residues and a thrombin cleavage sequence to the N-terminus to facilitate protein purification. Expression in Escherichia coli stain BL21(DE3)pLysS 701095
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