EC Number   |
|---|
    2.3.1.169 | expression in Escherichia coli |
| 2.3.1.169 | expression of a 49 kDa fragment containing residues 311-729 of the intact enzyme and a C-terminal His tag, in Escherichia coli |
| 2.3.1.169 | expression of ACSD beta in Escherichia coli strain NM522 |
| 2.3.1.169 | expression of C-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) from a pet29a(+) vector |
| 2.3.1.169 | gene ascB, expression of full-length protein, designated ACSCh732 amino acids, and as a form lacking the 317-amino acid N-terminal domain, designated ACSChDELTAN, 415 amino acids, in Escherichia coli strain NM522 |
| 2.3.1.169 | the gene has been cloned into Escherichia coli and found to be within an 11 kb gene cluster, recombinant enzyme is inactive |
| 2.3.1.169 | two putative operons encoding CODH/ACS, designated cdh1 (ma1016-ma1011) and cdh2 (ma3860-ma3865), both encoding bona fide CODH/ACS isoforms, as well as cdhA3 (ma4399), isogenes organisation, expression of the Cdh2-encoding genes is generally higher than that of genes encoding Cdh1, expression of cdh1, cdh2, and cdhA1 in Escherichia coli |
