EC Number   |
|---|
    2.3.1.167 | - |
| 2.3.1.167 | expression in Escherichia coli |
| 2.3.1.167 | gene dbat, functional expression in Escherichia coli strain JM109, co-expression with acetyl-CoA synthase, gene acs, EC 6.2.1.1, or with acetoacetate:acetyl-CoA CoA-transferase, genes atoAD of the ato operon, EC 2.8.3.8, expressed from and regulated by genes encoded on the bacterial chromosome, overview |
| 2.3.1.167 | into the TA cloning vector pMD19-T for sequencing |
| 2.3.1.167 | into the vector pCWori+ for expression in Escherichia coli JM109 and BL21DE3 cells |
| 2.3.1.167 | overexpression in Escherichia coli. Three kinds of fusion systems with isopropyl-beta-D-1-thiogalactopyranoside inducible promoters are used. The fusion partners are glutathione S-transferase tag, hexahistidine fusion tag, and a biotinylated peptide tag |
| 2.3.1.167 | the amplified PCR fragment is subcloned into the pGEM-T Easy vector and subsequently into the pET32a+ vector for expression in Escherichia coli BL21DE3 cells |
