EC Number |
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2.3.1.158 | DNA sequence determination and analysis, functional overexpression in Arabidopsis thaliana roots and leaves |
2.3.1.158 | expressed in Pichia pastoris |
2.3.1.158 | expression in Escherichia coli, expression in Pichia pastoris of a N-terminal deleted version of PDAT, lacking the predicted membrane-spanning region under the control of the methanol inducible alcohol oxidase promoter |
2.3.1.158 | expression in Saccharomyces cerevisiae |
2.3.1.158 | expression in tobacco leaves. The gene shows phospholipid diacylglycerol acyltransferase enzymatic activity and substantially increased triacylglycerol accumulation in the leaves |
2.3.1.158 | expression in tobacco leaves. The gene shows phospholipid diacylglycerol acyltransferase enzymatic activity and substantially increased triacylglycerol accumulation in the leaves. CsPDAT1-A shows a higher preference for alpha-linolenic acid (18:3 omega-3) |
2.3.1.158 | gene encoding PDAT, DNA and amino acid sequence determination and analysis, phylogenetic tree, expression of full-length enzyme and of truncated PDAT lacking the transmembrane domain in Pichia pastoris |
2.3.1.158 | gene LRO1, DNA and amino acid sequence determination and analysis |
2.3.1.158 | gene MiPDAT, DNA and amino acid sequence determination and analysis, quantitative RT-PCR enzyme expression analysis, recombinant expression of GFP-tagged enzyme in Nicotiana benthamiana leaves via transfection method with Agrobacterium tumefaciens, subcloning in Escherichia coli strain DH5alpha, recombinant expression of PDAT in TAG-deficient Saccharomyces cerevisiae mutant strain H1246, complementation and lipid analysis, overview |
2.3.1.158 | gene pdat or YALI0E16797g, DNA and amino acid sequence determination and analysis. In vivo expression of DGAT1, DGAT2 and PDAT under the heterologous Yarrowia lipolytica YAT promoter results in 598%, 702% and 278% increases in total fatty acid % dry cell weight over the empty vector control |