EC Number |
---|
2.1.1.74 | expressed in Escherichia coli as a His-tagged fusion protein |
2.1.1.74 | expression in Escherichia coli |
2.1.1.74 | overexpression in Escherichia coli strain BL21(DE3) |
2.1.1.74 | the encoded recombinant protein contains tightly bound flavin and is active in Escherichia coli mutant lacking m5U-54 in tRNAs and in vitro using T7 tRNA transcript as substrate |
2.1.1.74 | untagged N-terminus and C-terminus (His)6-tagged TrmFO from Bacillus subtilis is expressed in Escherichia coli. The tag does not significantly alter the expression level, flavin content, activity and secondary structure of the protein |