EC Number   |
|---|
    2.1.1.201 | BoCOQ5-2 partially complements the yeast coq5 mutant. Escherichia coli cells expressing BoCOQ5-2 exhibit a greater than 3fold increase in total ubiquinone content in comparison with the vector-only control |
| 2.1.1.201 | expression in Escherichia coli |
| 2.1.1.201 | gene COQ5 or YML110C, recombinant expression of N-terminally His-tagged truncated enzyme Coq5-DELTAN26 in Escherichia coli BL21(DE3), selenomethionine-labelled Coq5 protein is overexpressed in Escherichia coli strain B834 (DE3) |
| 2.1.1.201 | the COQ5 gene, when introduced into Escherichia coli, complements the respiratory deficiency of an ubiE mutant that maps near o251, suggesting that it is the yeast homolog of the ubiE gene product |
| 2.1.1.201 | the gene encoding human COQ5 spans to 26 kb on chromosome 12q24.31 and is comprised of seven exons, HEK-293 cells are co-transfected with the pCOQ5-myc and pCOQ4-V5, expression of a COQ5-GFP construct in HeLa cells, construction of a hybrid yeast-human COQ5 (yhCOQ5) gene by amplifying a 5' segment of yCOQ5, corresponding to the mitochondrial targeting region (encoding aa 1-54) and the 3' of human COQ5 |
