EC Number |
---|
2.1.1.179 | - |
2.1.1.179 | cloned in Streptomyces lividans. The sgm gene is expressed in Micromonospora melanosporea, where its own promoter is active, and also in Escherichia coli under the control of the lacZ promoter |
2.1.1.179 | cloning of the sgm gene into pET-25b (+) vector with the addition of N-terminal non-cleavable His6 tag, as well as alanine mutagenesis of residues D156, D182 and R108. The constructs are co-transformed along with pGroESL into the strain BL21 (DE3) of Escherichia coli for protein expression |
2.1.1.179 | expressed in Escherichia coli BL21(DE3) cells |
2.1.1.179 | expression in Escherichia coli |
2.1.1.179 | expression in Escherichia coli, Bacillus subtilis or Staphylococcus aureus |
2.1.1.179 | gene fmrO, phylogenetic analysis, sequence comparisons |
2.1.1.179 | gene grmA, phylogenetic analysis, sequence comparisons |
2.1.1.179 | gene grmB, phylogenetic analysis, sequence comparisons |
2.1.1.179 | gene kmr, phylogenetic analysis, sequence comparisons |