EC Number   |
|---|
    2.1.1.140 | canadine-producing Saccharomyces cerevisiae strain harbors expression cassettes for seven heterologous enzymes: Papaper somniferum norcoclaurine 6-O-methyltransferase (Ps6OMT), Papaver somniferum 3'-hydroxy-N-methylcoclaurine 4'-O-methyltransferase 2 (Ps4'OMT), Papapver somniferum coclaurine N-methyltransferase (PsCNMT), Papaver somniferum berberine bridge enzyme (PsBBE), Thalictrum flavum scoulerine 9-O-methyltransferase (TfS9OMT), Thalictrum flavum canadine synthase (TfCAS), and Arabidopsis thaliana cytochrome P450 reductase 1 (CPR). The expression cassettes for the methyltransferases Ps6OMT, PsCNMT, and Ps4'OMT and the cytochrome P450 reductase CPR were chromosomally integrated, TfS9OMT and TfCAS are expressed from a high-copy plasmid, and PsBBE is expressed from a second high-copy plasmid |
| 2.1.1.140 | expressed in Escherichia coli |
| 2.1.1.140 | expressed in Escherichia coli from the Salmonella enterica prpBCDE promoter, comparison to expression products derived from the strongest IPTG-inducible promoter PT7, DH10B strain used for DNA-manipulations, BLRDE3 strain host for pPro and T7 expression systems |
| 2.1.1.140 | expressed in Escherichia coli, recombinant CNMT protein |
| 2.1.1.140 | expression in Escherichia coli |
| 2.1.1.140 | isolation of molecular clones |
| 2.1.1.140 | real-time quantitative PCR analysis of target gene transcript levels, comparison of target gene transcript levels and papaverine content in individual opium poppy plants infiltrated with Agrobacterium tumefaciens harboring control and gene-specific pTRV2 constructs |
