EC Number   |
|---|
    2.1.1.116 | canadine-producing Saccharomyces cerevisiae strain harbors expression cassettes for seven heterologous enzymes: Papaper somniferum norcoclaurine 6-O-methyltransferase (Ps6OMT), Papaver somniferum 3'-hydroxy-N-methylcoclaurine 4'-O-methyltransferase 2 (Ps4'OMT), Papapver somniferum coclaurine N-methyltransferase (PsCNMT), Papaver somniferum berberine bridge enzyme (PsBBE), Thalictrum flavum scoulerine 9-O-methyltransferase (TfS9OMT), Thalictrum flavum canadine synthase (TfCAS), and Arabidopsis thaliana cytochrome P450 reductase 1 (CPR). The expression cassettes for the methyltransferases Ps6OMT, PsCNMT, and Ps4'OMT and the cytochrome P450 reductase CPR were chromosomally integrated, TfS9OMT and TfCAS are expressed from a high-copy plasmid, and PsBBE is expressed from a second high-copy plasmid |
| 2.1.1.116 | full-length cDNA of Coptis japonica 4'OMT is cloned into the binary vector pBITXEl2 for introduction into Agrobacterium tumefaciens, for infection Eschscholzia californica seedlings are co-cultured with Agrobacterium tumefaciens |
| 2.1.1.116 | into pRSETB and introduced into Escherichia coli strain ER2566 pLys S |
| 2.1.1.116 | into the pET-21d vector for expression in Escherichia coli BL21DE3 cells |
| 2.1.1.116 | into the pET-41a+ vector for expression in Escherichia coli BL21DE3 cells |
| 2.1.1.116 | into vector pQE31 containing six histidines as an N-terminal tag and overexpressed in Escherichia coli SG13009 |
| 2.1.1.116 | isolation of molecular clone |
