EC Number   |
|---|
    1.8.7.1 | expressed in Escherichia coli |
| 1.8.7.1 | expressed in Escherichia coli BL21(DE3) cells |
| 1.8.7.1 | expression in Escherichia coli |
| 1.8.7.1 | gene sir, expression from genomic DNA, wild-type FdSiR (FdSiRWT) and a mutant C491G (FdSiRC491G) are cloned from Synechococcus elongatus PCC 7942 and coexpressed with the cysG gene encoding siroheme synthase from Salmonella typhimurium using the pCDFDuet plasmid in Escherichia coli strain BL21(DE3) as His-tagged proteins. Recombinant expression of Synechococcus elongatus PCC 7942 ferredoxin 1 (Fd1) |
| 1.8.7.1 | into the vector pTrc99A for expression in Escherichia coli cells |
