EC Number |
---|
1.7.2.4 | cloning of the nos gene cluster |
1.7.2.4 | codon-optimized gene nosZ, from canonical gene cluster nosRZDFYL(X), recombinant overexpression of His6-tagged enzyme and of Strep-tagged enzyme in Escherichia coli strain BL21(DE3) |
1.7.2.4 | expressed in Escherichia coli |
1.7.2.4 | expressed in Escherichia coli BL21(DE3) cells |
1.7.2.4 | expression as metal-free apo protein |
1.7.2.4 | expression in Escherichia coli |
1.7.2.4 | expression in Escherichia coli HB101, no activity |
1.7.2.4 | expression in Nicotiana tabacum |
1.7.2.4 | gene nosZ, DNA and amino acid sequence determination and analysis, phylogenetic analysis, quantitative reverse transcriptase PCR expression analysis |
1.7.2.4 | gene nosZ, enzyme expression analysis during agricultural waste composting process, overview |