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EC Number Cloned (Commentary) Reference
Display the word mapDisplay the reaction diagram Show all sequences 1.7.1.B1expressed in Escherichia coli Rosetta pLysS cells 765362
Display the word mapDisplay the reaction diagram Show all sequences 1.7.1.B1For His-tagged protein production, plasmid is transformed into Escherichia coli BL21 pLysS. 692112
Display the word mapDisplay the reaction diagram Show all sequences 1.7.1.B1xenB gene is amplified using appropriate primers with BamHI and HindIII sites and Pseudomonas putida KT2440 chromosomal DNA as a template. After digestion with restriction enzymes, the PCR product is ligated into the pET28b(+) vector. Resulting plasmid contains the coding sequence in frame with a DNA sequence encoding a His-tag, which resulted in a hexahistidine tail. For protein-His6 expression, plasmid is transformed into Escherichia coli BL21. 690550
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