EC Number |
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1.7.1.3 | construction of a fusion gene comprising gene the promotor of gene niaD and the Escherichia coli beta-glucuronidase gene, i.e. GUS, for analysis of promotor activity in Aspergillus nidulans |
1.7.1.3 | expressed in Pichia pastoris |
1.7.1.3 | expression in Escherichia coli |
1.7.1.3 | gene nar1, DNA and amino acid sequence determination and analysis, expression study, gene nar1 is part of the nitrate assimilation gene cluster together with genes encoding a high-affinity nitrate transporter and a nitrite reductase, the intergenic region could also act as bidirectional promotor, organisation of coding sequences |
1.7.1.3 | gene tbnr1, DNA and amino acid sequence determination and analysis, expression analysis |
1.7.1.3 | gene tpnr1, DNA sequence determination and analysis, construction of a cDNA library |
1.7.1.3 | sequence comparisons, recombinant enzyme expression of wild-type and mutant enzymes in Escherichia coli strains TP1000, RK5206, and RK5204 |
1.7.1.3 | wild-type and mutants are expressed in Escherichia coli JM109(DE3)pLysS |