EC Number   |
Reference |
|---|
    1.5.8.4 | development of an intracellular, heterologous expression system in Komagataella phaffii (formerly known as Pichia pastoris). Initial attempts to express the gene in Escherichia coli (BL21 DE3) yielded largely insoluble protein. The H109R variant is expressed in lower amounts compared to the wild-type enzyme |
742499 |
| 1.5.8.4 | expressed in Escherichia coli BL21(DE3) cells as a C-terminally 6-His-tagged fusion protein which is predominantly in its apo form |
687135 |
| 1.5.8.4 | expressed in Komagataella phaffii KM71H cells |
742499 |
| 1.5.8.4 | expression in Escherichia coli |
699373, 763963 |
| 1.5.8.4 | expression in Komagataella phaffii, i.e. Pichia pastoris |
742499 |
| 1.5.8.4 | overexpression in Escherichia coli strain JM109 as N-terminally His-tagged enzyme being equally distributed in the soluble fraction and the insoluble inclusion body fraction |
657277 |
| 1.5.8.4 | screening of a cDNA library prepared in Escherichia coli from rat liver polyA RNA in the plasmid vector peD-X |
392524 |
