EC Number   |
|---|
  1.3.99.28 | expression in Escherichia coli |
| 1.3.99.28 | gene crtI, cloning and expression of wild-type and mutant enzymes in Escherichia coli strain DH5alpha |
| 1.3.99.28 | gene crtI, cloning of the crt gene cluster crtCDEF, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) |
| 1.3.99.28 | gene crtI, phylogenetic tree, co-expression with 5 other enzymes of the carotenoid pathway from Pantoea agglomerans, i.e. IPP isomerase, FPP synthase, GGPP synthase, phytoene synthase, lycopene cyclase, beta-carotene hydrolase, and zeaxanthin glucosyltransferase, in Escherichia coli, functional complementation by CrtI of Brevibacterium linens, CGI, CrtI of Corynebacterium glutamicum, RSI, CrtI of Rhodobacter sphaeroides RCI, CrtI of Rhodobacter capsulatus, and RBI, CrtI of Rhodopirellula baltica, and the homologous complementation of CrtI from Pantoea agglomerans with the Pantoea agglomerans carotenogenic module expressing CrtEPAG -CrtBPAG |
| 1.3.99.28 | gene crtI, sequence comparisons and phylogenetic analysis, recombinant expression in Escherichia coli |
| 1.3.99.28 | sequence comparisons and phylogenetic analysis, recombinant expression in Escherichia coli |
| 1.3.99.28 | the phytoene desaturase gene, from Rhodobacter capsulatus is functionally complemented with a gene construct from Erwinia uredovora which encodes all enzymes responsible for formation of 15-cis phytoene in Escherichia coli |
