EC Number   |
|---|
   1.3.7.4 | - |
| 1.3.7.4 | development of a system to produce phytochromobilin in Escherichia coli by coexpression of heme oxygenase and phytochromobilin:ferredoxin oxidoreductase in a single operon in conjunction with apophytochrome using two compatible plasmids |
| 1.3.7.4 | expressed in Escherichia coli strain BL21 |
| 1.3.7.4 | expression in Nicotiana plumbaginifolia and Escherichia coli |
| 1.3.7.4 | expression in Pichia pastoris |
| 1.3.7.4 | gene AUREA (AU), quantitative real-time RT-PCR enzyme expression analysis in wild-type and mutant seedlings |
| 1.3.7.4 | gene CsHY2, genotyping, several segregating populations qre employed by crossing C1238 with the original parental line CCMC, the American pickling cucumber line Gy14 and the North China fresh marker type cucumber line 9930, respectively. For linkage mapping and cloning of the elh1 gene, F2 populations are derived from the C1238×9930 F1 and C1238×Gy14 F1 plants, respectively. Quantitative real-time PCR enzyme expression analysis. Phylogenetic analysis of CsHY2 homologues from different plants. Linkage mapping of the elh1 locus |
| 1.3.7.4 | overexpression in Synechococcus sp. strain PCC 7002, from endogenous plasmid pAQ1 under the control of the Synechocystis sp. strain PCC 6803 cpcBA promoter, leads to overproduction of phytochromobilin, the cells show a phenotype only slightly less pigmented and blue-green than the wild-type, the strain producing phycobiliproteins carrying phytochromobilin grow much more slowly at low light intensity. Transformant colonies in which pcyA is inactivated in the HY2 overexpression background does not develop a chlorotic appearance, and segregation of the mutant and wild-type alleles is rapidly achieved |
| 1.3.7.4 | recombinant functional expression of HT-HY2 in Escherichia coli with production of phytochromobilin, functional co-expression with cyanobacterial heme oxygenase, and the phycocyanin alpha-subunit, CpcA, from Synechocystis sp. PCC 6803 or Synechococcus sp. PCC 7002, and with the phycocyanin alpha-subunit phycocyanobilin lyase, CpcE/CpcF, or the phycoerythrocyanin alpha-subunit phycocyanobilin isomerizing lyase, PecE/PecF, from Noctoc sp. PCC 7120. Production levels of fluorescent pigments and chromophore analysis, overview |
| 1.3.7.4 | recombinant overexpression of His-tagged enzyme in Escherichia coli strain BL21(DE3) |
