EC Number   |
|---|
   1.3.7.3 | - |
| 1.3.7.3 | expressed using a tac promoter-driven N-terminal GST fusion protein |
| 1.3.7.3 | expression in Escherichia coli |
| 1.3.7.3 | gene pebB, in Synechococcus sp. WH8020, the genes encoding for pebA and pebB share an overlapping region. The pebA stop codon TGA is part of the pebB start codon ATG, recombinant expression of a translational fusion between pebA and pebB, N-terminally Strep-tagged and C-terminally His-tagged PebAgB, recombinant expression of GST-tagged or His-tagged PebB in Escherichia coli BL21(DE3) |
| 1.3.7.3 | gene pebB, recombinant expression of the codon optimized gene, without signal peptide, in Escherichia coli, expression of GST-tagged wild-type and mutant enzymes in Escherichia coli |
| 1.3.7.3 | gene pebB, recombinant expression of wild-type and mutant enzymes |
| 1.3.7.3 | into pASK-IBA45+ and transformed in Escherichia coli DH10B cells |
| 1.3.7.3 | into vector pKT270, co-expression with cyanobacterial phytochrome 1 in Escherichia coli JM109 |
