1.3.3.8 | DNA and amino acid sequence determination and analysis, functional ectopical expression of CjTHBO under the control of Cauliflower mosaic virus 35S promoterin Eschscholzia californica cells via transformation with Agrobacterium tumefaciens strain LBA4404. The transgenic cells produce coptisine and dehydrocheilanthifoline, which are expected to be generated by the reaction of CjTHBO from pathway intermediates, i.e. cheilanthifoline and stylopine, alkaloid profile analysis in transgenic California poppy cells, overview. Expression in Escherichia coli strain BL21 (DE3) |
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