EC Number |
---|
1.2.1.31 | - |
1.2.1.31 | cDNA is cloned from a human fetal library, library is constructed in a modified pBluescript II SK+ vector, cDNA insert is sequenced |
1.2.1.31 | cloning of lys2, the cloned gene encodes a functional alpha-aminoadipate reductase as shown by complementation of a Penicillium chrysogenum lys2 mutant, the aminoacid sequence shows strong homology with the alpha-aminoadipate reductase encoded by lys2 genes of Candida albicans, Schizosaccharomyces pombe, Saccharomyces cerevisiae and Penicillium chrysogenum |
1.2.1.31 | cloning of the LYS2 gene |
1.2.1.31 | encoded by two different genes, LYS2 and LYS5 |
1.2.1.31 | expression in Escherichia coli as an active enzyme. Exhibits significant alpha-aminoadipate reductase activity without the addition of CoA and Lys7p PPTase from Schizosaccharomyces pombe |
1.2.1.31 | heterlogous expressed in Escherichia coli |
1.2.1.31 | LYS2 gene cloned |
1.2.1.31 | LYS2 gene codes for alpha-aminoadipate reductase, construction and use of LYS2 cartridges |
1.2.1.31 | LYS2 gene codes for alpha-aminoadipate reductase, LYS2 genes encodes a protein with 49.9% identity to the Saccharomyces cerevisiae, 51.3% identity to the Schizosaccharomyces pombe and 48.12% identity to the Candida albicans alpha-aminoadipate reductases |