EC Number   |
Reference |
|---|
    1.2.1.10 | - |
390179, 724341 |
| 1.2.1.10 | Escherichia coli strain W1485 |
390173 |
| 1.2.1.10 | expressed in Escherichia coli C41 (DE3) cells |
724721 |
| 1.2.1.10 | expression in Escherichia coli |
735506, 735567, 736748 |
| 1.2.1.10 | expression in Escherichia coli BL21 and DH5alpha |
672735 |
| 1.2.1.10 | expression in Pyrococcus furiosus from which the native aldehyde oxidoreductase (AOR) gene is deleted. A strain containing the Thermoanaerobacter ethanolicus AdhE in a synthetic operon with AdhA is constructed. The AdhA gene is amplified from Thermoanaerobacter sp. X514. The amino acid sequence of AdhA from Thermoanaerobacter sp. X514 is identical to that of AdhA from Thermoanaerobacter ethanolicus. Of the bacterial strains expressing the various heterologous AdhE genes, only those containing AdhE and AdhA from Thermoanaerobacter sp. produced ethanol above background. The Thermoanaerobacter ethanolicus AdhEA strain containing both AdhE and AdhA produces the most ethanol (4.2 mM), followed by Thermoanaerobacter ethanolicus AdhE strain (2.6 mM), Thermoanaerobacter ethanolicus AdhA strain (1.8 mM) and Thermoanaerobacter sp. X514 AdhE strain (1.5 mM). Ethanol and acetate are the only major carbon end-products from glucose under these conditions. For these four strains, the amount of ethanol produced per estimated glucose consumed is increased from the background level to 1.2, 1.0, 0.8 and 0.7 respectively |
738990 |
| 1.2.1.10 | expression of the enzyme from Thermoanaerobacter sp. X514 in Pyrococcus furiosus from which the native aldehyde oxidoreductase (AOR) gene is deleted. Ethanol and acetate are the only major carbon end-products from glucose under these conditions. The amount of ethanol produced per estimated glucose consumed is increased from the background level 0.7 respectively |
738990 |
| 1.2.1.10 | gene adhE, phylogenetic analysis, quantitative RT-PCR enzyme expression analysis |
763253 |
| 1.2.1.10 | gene adhE, pyruvate-formate-lyase-deactivase with alcohol and acetaldehyde dehydrogenase activity |
390163 |
| 1.2.1.10 | gene adhe, recombinant expression of N-terminally His6-tagged AdhE in Escherichia coli strain Bl21 |
763497 |
