EC Number   |
|---|
   1.14.99.66 | coexpression of HA-tagged SALL4 and FLAG-tagged LSD1 in HEK-293 cells, coexpression of LSD1 and SALL4 proteins in mouse bone marrow hematopoietic stem and progenitor cells |
| 1.14.99.66 | determination of expression of Aof1 wild-type and hetero- or homozygous mutants during development. Functional expression of Flag-tagged wild-type KDM1B and mutant KDM1B in nuclei of HEK 293T cells and NIH 3T3 cells |
| 1.14.99.66 | expression in Escherichia coli |
| 1.14.99.66 | expression of His6-tagged LSD1 in Escherichia coli strain BL21(DE3) |
| 1.14.99.66 | fragment consisting of residues 172-833, expression as glutathione S-transferase fusion protein in Escherichia coli |
| 1.14.99.66 | gene KDM1A, codon optimized, recombinant expression of the enzyme LSD1, residues 151-852, in Escherichia coli |
| 1.14.99.66 | gene KDM1A, quantitative real-time PCR enzyme expression analysis |
| 1.14.99.66 | gene KDM1A, recombinant expression of FLAG-tagged wild-type and mutant enzymes in Escherichia coli strain BL21, recombinant expression of FLAG-tagged wild-type enzyme in Spodoptera frugiperda SF21 cells via baculovrial expression system. Ectopic expression of the LSD1 enzymatically dead mutant K661A |
| 1.14.99.66 | gene KDM1A, recombinant expression of full-length His6-tagged human LSD1 from pET15b bacterial expression vector in Escherichia coli strain BL21(DE3) |
| 1.14.99.66 | gene KDM1A, recombinant expression of N-terminally His-tagged full-length LSD1 protein and deletion mutant DELTA184 LSD1lacking the N-terminal 184 amino acid residues |
