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EC Number Cloned (Commentary)
Display the reaction diagram Show all sequences 1.14.99.14expressed in Pichia pastoris GS115 cells
Display the reaction diagram Show all sequences 1.14.99.14expressed in Rhizopus nigericans
Display the reaction diagram Show all sequences 1.14.99.14gene CYP509C12, cloning from a cDNA library, DNA and amino acid sequence determination and analysis, phylogenetic analysis, and recombinant expression in Pichia pastoris, coexpression with NAD(P)H-dependent reductase also from Rhizopus oryzae, which improves electron transfer to CYP509C12 and thus increases the production of 11-hydroxyprogesterone by 7fold. The engineered strain displays total bioconversion of progesterone into 11alpha-hydroxyprogesterone and small amounts of 6beta-hydroxyprogesterone
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