EC Number   |
|---|
   1.14.19.64 | coexpression of cheilanthifoline synthase (CYP719A5), and stylopine synthase (CYP719A2) in Pichia pastoris. Biosynthetic enzyme expression is examined in a consolidated system with all genes expressed in one cell and a coculture system with three cell lines that each express a single gene were examined. Although both systems efficiently converted reticuline to stylopine, the consolidated system is more rapid and efficient than the co-culture system. However, substrate-feeding experiments reveal a decrease in the conversion efficiency in the consolidated system during successive cultures, whereas the conversion efficiency in the co-culture system remains constant. Thus, the final amount of stylopine produced from reticuline after successive feedings in the co-culture system is more than 150 nmoles from 750 nmoles of (R,S)-reticuline (375 nmoles of (S)-reticuline) |
| 1.14.19.64 | CYP719A2 and CYP719A3, DNA and amino acid sequence determination and analysis, expression patterns, expression in Saccharomyces cerevisiae microsomes |
| 1.14.19.64 | expressed in Spodoptera frugiperda Sf9 cells |
| 1.14.19.64 | heterologously expressed in Saccharomyces cerevisiae |
| 1.14.19.64 | two full-length P450 cDNAs, CYP719A2 and CYP7193A, expression in Saccharomyces cerevisiae |
