EC Number   |
Reference |
|---|
    1.14.19.45 | expressed in Bacillus subtilis |
733169 |
| 1.14.19.45 | expressed in Solanum tuberosum cultivar Desnitsa |
728791 |
| 1.14.19.45 | gene CsFAD2, variant FAD2-1, genetic structure, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative real-time PCR enzyme expression analysis, subcloning in Escherichia coli strain XL1-Blue, and functional recombinant expression in Saccharomyces cerevisiae strain W303-1A. The recombinant CsFAD2 shows conversion of 18:1DELTA9 to 18:2DELTA9,12, slightly better at 30°C than at 22°C, and it is also able to desaturate palmitoleic acid (16:1DELTA9) to hexadecadienoic acid (16:2DELTA9,12). Fatty acid composition of Saccharomyces cerevisiae cells overexpressing CsFAD2 microsomal desaturase and grown at two different temperatures, overview |
745061 |
| 1.14.19.45 | gene CsFAD2, variant FAD2-2, genetic structure, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative real-time PCR enzyme expression analysis, subcloning in Escherichia coli strain XL1-Blue, and functional recombinant expression in Saccharomyces cerevisiae strain W303-1A. The recombinant CsFAD2 shows conversion of 18:1DELTA9 to 18:2DELTA9,12, slightly better at 30°C than at 22°C, and it is also able to desaturate palmitoleic acid (16:1DELTA9) to hexadecadienoic acid (16:2DELTA9,12). Fatty acid composition of Saccharomyces cerevisiae cells overexpressing CsFAD2 microsomal desaturase and grown at two different temperatures, overview |
745061 |
| 1.14.19.45 | gene CsFAD2, variant FAD2-3, genetic structure, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative real-time PCR enzyme expression analysis, subcloning in Escherichia coli strain XL1-Blue, and functional recombinant expression in Saccharomyces cerevisiae strain W303-1A . The recombinant CsFAD2 shows conversion of 18:1DELTA9 to 18:2DELTA9,12, slightly better at 30°C than at 22°C, and it is also able to desaturate palmitoleic acid (16:1DELTA9) to hexadecadienoic acid (16:2DELTA9,12). Fatty acid composition of Saccharomyces cerevisiae cells overexpressing CsFAD2 microsomal desaturase and grown at two different temperatures, overview |
745061 |
| 1.14.19.45 | gene FAD2, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, overexpression in Saccharomyces cerevisiae strain BY4741 supplemented with linoleic acid (DELTA9,DELTA12-18:2) and hexadecadienoic acid (DELTA9,DELTA12-16:2) leads to accumulation of DELTA15-PUFAs, i.e., alpha-linolenic acid (DELTA9,DELTA12,DELTA15-18:3) and hexadecatrienoic acid with an unusual terminal double bond (DELTA9,DELTA12,DELTA15-16:3) results in production of a range of DELTA12- and DELTA15-PUFAs. The major products of CpFad2 are linoleic and hexadecadienoic acid (DELTA9,DELTA12-16:2), accompanied by alpha-linolenic acid and hexadecatrienoic acid (DELTA9,DELTA12,DELTA15-16:3). Ricinoleic acid (12-hydroxy-9-octadecenoic acid) is an additional product of CpFad2 identified by GC/MS analysis |
746264 |
| 1.14.19.45 | gene RKD12, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative real-time PCR enzyme expression analysis, recombinant expression in Saccharomyces cerevisiae |
744576 |
| 1.14.19.45 | heterologous expression in Saccharomyces cerevisiae. Plasmid pYES2-egFAD2 is transformed into Saccharomyces cerevisiae strain INVSc1 using lithium acetate method for expression under the induction of galactose |
745028 |
| 1.14.19.45 | overexpression in Saccharomyces cerevisiae |
746264 |
| 1.14.19.45 | recombinant expression of hybrid gene of Synechococcus vulcanus DELTA12 (desA) acyllipid desaturase in Nicotiana tabacum cv. Wisconsin, as chimeric enzyme DesA-LicBM3 with reporter thermostable lichenase of Clostridium thermocellum, expression with or without the sequence coding for transit peptide of Rubisco small subunit of Arabidopsis thaliana under control of the 35S CaMV constitutive promoter using the Agrobacterium tumefaciens strain GV3101-mediated transformation method, measurement of recombinant lichenase activity. Plants expressing RTP::desA::licBM3 gene coding for DELTA12 desaturase with the transit peptide for transport into chloroplasts causes a reliable increase of DELTA9,12-18:2 fatty acids and a decrease of DELTA9,12,15-18:3 fatty acids |
746429 |
