EC Number   |
|---|
   1.14.19.36 | gene des3-1, DNA and amino acid sequence determination and analysis, phylogenetic analysis, quantitative PCR expression analysis |
| 1.14.19.36 | gene desB, DNA and amino acid sequence determination and analysis, phylogenetic tree, genes desA, desB, and desC are in tandem and have an ORF of 822 bp, 1053 bp, and 1080 bp, respectively, and are separated by intergenic regions, the three genes do not form an operon, RT-PCR enzyme expression analysis |
| 1.14.19.36 | gene desB, DNA and amino acid sequence determination and analysis, sequence comparison, temperature-dependent expression, abundant expression at 22°C in cells, no expression at 38°C, steady-state levels overview. The half-life of desB mRNA is significantly longer in cells grown at 22°C than in cells grown at 38°C |
| 1.14.19.36 | gene FAD8, generation of FAD8-YFP overexpressing lines, that show a specific increase in 18:3 fatty acids at 22°C |
| 1.14.19.36 | the desA gene, which encodes the DELTA12 desaturase of Synechocystis sp. PCC 6803, and the desB gene are introduced into Synechococcus sp. PCC 7942. Whilst the parent cyanobacterium can only desaturate membrane lipids at the DELTA9 position of fatty acids, the resultant transformant is able to desaturate fatty acids of membrane lipids at the DELTA9, DELTA12 and omega3 positions |
