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Results 1 - 10 of 10
EC Number Cloned (Commentary)
Display the word mapDisplay the reaction diagram Show all sequences 1.14.19.28DNA and amino acid sequence determination and analysis, functional overexpression of His6-tagged enzyme in Escherichia coli strain BL21 codon Plus, subcloning in Escherichia coli strain XL 10 GOLD, TcDELTA9DES-expressing Escherichia coli cells exhibit high desaturase activity associated with increased levels of monounsaturated fatty acids. The fatty acid composition of Escherichia coli shows that the recombinant desaturase can use 16:0 as substrate
Display the word mapDisplay the reaction diagram Show all sequences 1.14.19.28expressed in Escherichia coli
Display the word mapDisplay the reaction diagram Show all sequences 1.14.19.28expressed in Nicotiana tabacum
Display the word mapDisplay the reaction diagram Show all sequences 1.14.19.28expressed in Nicotiana tabacum leaves
Display the word mapDisplay the reaction diagram Show all sequences 1.14.19.28expressed in Saccharomyces cerevisiae strain AMY2alpha
Display the word mapDisplay the reaction diagram Show all sequences 1.14.19.28expression in Escherichia coli
Display the word mapDisplay the reaction diagram Show all sequences 1.14.19.28gene desC1, DNA and amino acid sequence determination, phylogenetic analysis
Display the word mapDisplay the reaction diagram Show all sequences 1.14.19.28gene PA3FAD9, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, functional recombinant expression in Escherichia coli, GCMS analysis reveals that palmitoleic (C16:1DELTA9) and oleic acids (C18:1DELTA9) are the only unsaturated fatty acids identified in addition to the endogenous fatty acids, expression at 15°C
Display the word mapDisplay the reaction diagram Show all sequences 1.14.19.28gene PA8FAD9, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression in Escherichia coli strain Transetta (DE3), ability of the enzyme to convert exogenous stearic acid to oleic acid incorporated into the recombinant Escherichia coli membrane phospholipids. GCMS analysis confirms significant changes in the pattern of palmitoleic and palmitic acid production in Escherichia coli cells expressing PA8FAD9 gene. At 20°C, the amount of palmitoleic increased from 10.5 to 21% suggesting that PA8FAD9 gene from Pseudomonas sp. A8 codes for an active DELTA9-fatty acid desaturase-like protein
Display the word mapDisplay the reaction diagram Show all sequences 1.14.19.28recombinant expression of hybrid gene of Synechococcus vulcanus DELTA9 (desC) acyl-lipid desaturase in Nicotiana tabacum cv. Wisconsin, as chimeric enzyme DesC-LicBM3 with reporter thermostable lichenase of Clostridium thermocellum, expression with or without the sequence coding for transit peptide of Rubisco small subunit of Arabidopsis thaliana under control of the 35S CaMV constitutive promoter using the Agrobacterium tumefaciens strain GV3101-mediated transformation method, measurement of recombinant lichenase activity. Plants expressing RTP::desC::licBM3 gene coding for DELTA9 desaturase with the transit pepride for transport into chloroplasts exhibit a significant increase of alpha-linolenic acid (DELTA9,12,15-18:3) portion accompanied by decrease of DELTA9,12-18:2 level while the part of the third major fatty acid, palmitic acid, remains unchanged
Results 1 - 10 of 10