EC Number   |
|---|
   1.14.18.3 | - |
| 1.14.18.3 | DNA and amino acid sequence determination and anaylsis, the gene is organized in the structural gene cluster pmoCAB, stable functional expression of the membrane-bound enzyme in Rhodococcus erythropolis strain LSSE8-1 by using the dsz promoter and ethane as the sole carbon source, method optimization, overview |
| 1.14.18.3 | expressed in Escherichia coli |
| 1.14.18.3 | expressed in Escherichia coli BL21 cells |
| 1.14.18.3 | expressed in Escherichia coli BL21(DE3) or Rosetta (DE3) pLysS cells |
| 1.14.18.3 | gene pmoA, design of a highly degenerate primer targeting copper-containing membrane-bound monooxygenase genes for community analysis of methane- and ammonia-oxidizing bacteria, two-step PCR strategy employing a tagged highly degenerate primer (THDP), designated THDP-PCR, method development and optimization, overview. DNA and amino acid sequence determination and analysis, phylogenetic analysis |
| 1.14.18.3 | genetic analysis of pMMO genes |
| 1.14.18.3 | pmoA cloned in pGEM-T Easy vector and transformed into Escherichia coli JM109 high-efficiency competent cells |
| 1.14.18.3 | recombinant expression of wild-type holoenzyme and truncated recombinant periplasmic domains of pMMO (spmoB) |
| 1.14.18.3 | two aqueous-exposed subdomains toward the N- and C-termini of the large subunit are expressed in Escherichia coli BL21 (DE3) cells |
