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EC Number Cloned (Commentary)
Display the word mapDisplay the reaction diagram Show all sequences 1.14.14.99-
Display the word mapDisplay the reaction diagram Show all sequences 1.14.14.99expression in Escherichia coli
Display the word mapDisplay the reaction diagram Show all sequences 1.14.14.99expression in Escherichia coli and Saccharomyces cerevisiae
Display the word mapDisplay the reaction diagram Show all sequences 1.14.14.99expression of the chimeric protein in Escherichia coli
Display the word mapDisplay the reaction diagram Show all sequences 1.14.14.99overexpression in Pichia pastoris. The enzyme shows significantly enhanced activity upon RAD52 over-expression and cultivation optimization. (-)-Limonene hydroxylation is 2fold increased by RAD52 overexpression in strains PpPM17/AtCPR, expressing limonene-3-hydroxylase (PM17 isoform) and Arabidopsis thaliana cytochrome P450 reductase (CPR), while cultivation at pH 8 yields in about 55% increased isopiperitenol formation. The two effects are largely additive leading to an about 2.5fold enhanced isopiperitenol production in PpPM17/CPR-RAD52 over the starting strain/condition
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