EC Number |
---|
1.14.14.3 | - |
1.14.14.3 | amplified from the pJHD500 plasmid and ligated into a pET21b vector, expressed from pZCH2 in an Escherichia coli BL21 (lambdaDE3) cell line after growth to an OD600 of 0.5 |
1.14.14.3 | coexpression of luciferase and cytochrome P-450 |
1.14.14.3 | expressed in Escherichia coli BL21(DE3) and HEK-293T cells |
1.14.14.3 | expressed in Escherichia coli BL21(DE3) cells |
1.14.14.3 | expressed in Escherichia coli from pJHD500, ligated into a pET21b vector. Luciferase subcloned from pZCH2 into a pASKIBA-3c vector with the restriction sites XbaI and XhoI. The resulting luciferase containing a strep-II tag on the C terminus of the beta-subunit (pZCB4) expressed |
1.14.14.3 | expressed in Escherichia coli JM109 (native enzyme) and BL21(DE3) cells (luciferase-mOrange fusion enzyme) |
1.14.14.3 | expressed in NIH-3T3 cells |
1.14.14.3 | expressed in Pseudomonas putida mt-2 |
1.14.14.3 | expression analysis, cloning into expression vector pPL2lux useable as a reporter system based on the luciferase activity of the enzyme, overview |