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EC Number	Cloned (Commentary)	Reference
1.14.13.180	-	722484, 722486
1.14.13.180	expression in doxorubicin-sensitive Streptomyces galilaeus ATCC 31133. Transformation of Streptomyces galilaeus ATCC 31133 with plasmid pMC213 containing the aklavinone 11-hydroxylase gene (dnrF) results in the production of many red pigments. A new metabolite is purified, and the position of the newly introduced hydroxyl group is determined. The result indicates that the aklavinone 11-hydroxylase gene is stably expressed in Streptomyces galilaeus ATCC 31133 and that it gives rise to a hybrid aclacinomycin A which shows highly specific in vitro cytotoxicity against leukemia and melanoma cell lines	721298
1.14.13.180	heterologous expression of dnrF in Streptomyces liwidans TK23 and in Escherichia coli. Since the fusion protein does not show any hydroxylase activity, the dnrF gene is cloned into the pT7-7 vector that allows the expression of an unfused DnrF protein	723117
1.14.13.180	overexpression of the gene in Streptomyces lividans	721697

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Release 2023.1 (January 2023)