EC Number   |
|---|
   1.14.13.167 | expressed in Escherichia coli BL21(DE3) cells |
| 1.14.13.167 | expressed in Escherichia coli Rosetta(DE3)pLysS cells |
| 1.14.13.167 | gene pnpA, expression of the pnp cluster is detected only under inducing conditions, and pnpABA1CDEFG is a single transcriptional operon, genetic structure, RT-PCR and real-time PCR enzyme expression analysis, recombinant expression of N-terminally His6-tagged enzyme PnpA in Escherichia coli strain Rosetta(DE3)pLysS |
| 1.14.13.167 | gene pnpA1, recombinant expression of N-terminally His6-tagged enzyme PnpA in Escherichia coli strain Rosetta(DE3)pLysS |
| 1.14.13.167 | His-tagged enzyme is expressed in Escherichia coli BL21(DE3) cells |
| 1.14.13.167 | the enzyme is encoded in the pnpABA1CDEF cluster, which is involved in para-nitrophenol (PNP) and 2-chloro-4-nitrophenol (2C4NP) catabolism and is also likely responsible for 3-methyl-4-nitrophenol (3M4NP) degradation in strain SJ98, real-time quantitative PCR enzyme expression analysis, recombinant expression of N-terminally His6-tagged enzyme PnpA in Escherichia coli strain Rosetta(DE3)pLysS |
