EC Number   |
Reference |
|---|
   1.14.11.6 | DNA and amino acid sequence determination and analysis, expression of GST-tagged enzyme in Escherichia coli strain BL21(DE3) |
697315 |
| 1.14.11.6 | expressed in Escherichia coli BL21(DE3)Gold |
658220 |
| 1.14.11.6 | expression in Escherichia coli, fusion protein with green fluorescent protein and with His-tag |
689246 |
| 1.14.11.6 | expression of either the wild type JBP2 or the mutant JBP2 proteins in insect stage trypanosomes, which do not contain the modified base J and determined the base J content of each cell line, mutations made within the key catalytic domain ablates the ability of JBP2 to initiate de novo base J synthesis |
700501 |
| 1.14.11.6 | JBP2, DNA and amino acid sequence determination and analysis, expression of wild-type and mutant enzymes, the latter contain 4 exchanges of conserved residues |
700084 |
| 1.14.11.6 | recombinant expression of C-terminally His6-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) Codon Plus |
745921 |
