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Results 1 - 10 of 10
EC Number Cloned (Commentary) Reference
Show all pathways known for 1.12.1.3Display the word mapDisplay the reaction diagram Show all sequences 1.12.1.3- 439690, 659019
Show all pathways known for 1.12.1.3Display the word mapDisplay the reaction diagram Show all sequences 1.12.1.3construction of a strain of Pyrococcus furiosus that contains a second set of the eight genes involved in the maturation of the catalytic subunit and insertion of the [NiFe]-site, along with a second set of the four genes encoding the NADPH-dependent cytoplasmic [NiFe]-hydrogenase (SHI) structural subunits. This results in a 40% higher yield of the purified affinity-tagged enzyme and the content of the inactive trimeric form decreases to 5% of the total protein 765702
Show all pathways known for 1.12.1.3Display the word mapDisplay the reaction diagram Show all sequences 1.12.1.3expression in Escherichia coli. The native Escherichia coli maturation machinery will also generate a functional hydrogenase when provided with only the genes encoding the hydrogenase subunits and a single protease from Pyrococcus furiosus. The expression is induced by anaerobic conditions, whereby Escherichia coli is grown aerobically and production of recombinant hydrogenase is achieved by simply changing the gas feed from air to an inert gas (N2). The recombinant enzyme is purified and shown to be functionally similar to the native enzyme purified from Pyrococcus furiosus. The methodology to generate this key hydrogen-producing enzyme has dramatic implications for the production of hydrogen and NADPH as vehicles for energy storage and transport, for engineering hydrogenase to optimize production and catalysis, as well as for the general production of complex, oxygen-sensitive metalloproteins 746235
Show all pathways known for 1.12.1.3Display the word mapDisplay the reaction diagram Show all sequences 1.12.1.3expression of C-terminal domain of subunit HndA and N-terminal domain of subunit HndD in Escherichia coli 677009
Show all pathways known for 1.12.1.3Display the word mapDisplay the reaction diagram Show all sequences 1.12.1.3HndA and HndB subunit 439687
Show all pathways known for 1.12.1.3Display the word mapDisplay the reaction diagram Show all sequences 1.12.1.3HndA and HndD subunit 439688
Show all pathways known for 1.12.1.3Display the word mapDisplay the reaction diagram Show all sequences 1.12.1.3non-lethal deletions 439688
Show all pathways known for 1.12.1.3Display the word mapDisplay the reaction diagram Show all sequences 1.12.1.3overexpressed in Thermococcus kodakarensis KOD1 results in more than 1200-fold enhancement in the hydrogenase activity of the cell lysate compared to that of the host strain with an empty vector 764358
Show all pathways known for 1.12.1.3Display the word mapDisplay the reaction diagram Show all sequences 1.12.1.3overexpression in Synechocystis 704606
Show all pathways known for 1.12.1.3Display the word mapDisplay the reaction diagram Show all sequences 1.12.1.3the four gene operon encoding the enzyme is overexpressed in Pyrococcus furiosus and includes a polyhistidine affinity tag 730870
Results 1 - 10 of 10