EC Number |
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1.1.3.41 | DNA and amino acid sequence determination and analysis, sequence comparison, expression of His6-tagged enzyme in Escherichia coli strain MC1061 |
1.1.3.41 | expressed in Escherichia coli C41 (DE3) cells |
1.1.3.41 | expressed in Escherichia coli Top10 cells |
1.1.3.41 | expression in Escherichia coli |
1.1.3.41 | functional expression of recombinant oxidase-peroxidase fusion mutant enzyme in Escherichia coli strain BL21(DE3) periplasm |
1.1.3.41 | gene aldO, DNA and amino acid sequence determination and analysis, sequence comparison, expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) |
1.1.3.41 | recombinant enzyme expression in the periplasm or on the cell surface of Escherichia coli cells of different strains. The enzyme is differently tagged, i.e. expressed as Tat-AldO or INP-AldO, and exported to the periplasm or to the cell surface of the transformed cells, overview. AldO is successfully displayed at the surface of Escherichia coli using a truncated INP variant, it contains covalently bound FAD, thus has attained a correctly folded and active conformation |