EC Number |
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1.1.1.B19 | expressed in Saccharomyces cerevisiae strain PWY1113 |
1.1.1.B19 | expressed in Saccharomyces cerevisiae strain PWY2313 |
1.1.1.B19 | expression in Escherichia coli |
1.1.1.B19 | gene XDH, DNA and amino acid sequence determination and analysis, sequence comparisons |
1.1.1.B19 | gene XDH, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis |
1.1.1.B19 | gene XYL2, recombinant expression in Saccharomyces cerevisiae strain SCB7 resulting in strains SCF202 and SCF201, coexpression of XYL2 with XYL1, encoding a xylose reductase, and the endogenous XKS1, encoding a xylulokinase, all under the control of the TDH3 promoter in the chromosomal DNA, the recombinant strain efficiently grows in minimal medium containing xylose as the sole carbon source. An additional mutation of MTH1, encoding the endogenous negative regulator of the glucose-sensing signal transduction pathway, MTH1e32, and GRR1, encoding endogenous Grr1, an Fbox protein component of an SCF ubiquitineligase complex grr1e33, enable efficient growth in medium containing high xylose concentrations, subcloning in Escherichia coli strain DH10B |
1.1.1.B19 | recombinant expression in Bacillus subtilis, coexpression with the water-forming enzyme NADH oxidase (NOX) for NAD+ recycling leading to 3.4fold increased XDH activity |