EC Number   |
|---|
    1.1.1.79 | - |
| 1.1.1.79 | exclusive localization in the cytosol of transgenic Arabidopsis plants co-expressing GFP-GLYR1 and and Cherry-PTS1, a fusion protein consisting of the Cherry fluorescent protein linked to the PTS1 of the peroxisomal enzyme hydroxypyruvate reductase. Expression of N-terminal GFP-tagged or Myc-tagged GLYR1 in tobacco BY-2 cell cytosol. GFP- or Myc-tagged GLYR1 is competent, at least partially, for import into peroxisomes, since replacement of the C-terminal glutamate in GLYR1 with leucine, which yields a canonical PTS1 (i.e., a C-terminal small-basic-hydrophobic tripeptide motif), results in the modified fusion protein (GFPGLYR1-E to L and Myc-GLYR1-E to L) being dual localized to the cytosol and peroxisomes in BY-2 cells |
| 1.1.1.79 | expressed as GFP-fusion protein in tobacco BY-2 cells |
| 1.1.1.79 | expressed as His-tag fusion protein in E. coli BL-21(DE3) Rosetta (pLysS), full-length and truncated GR2 sequences introduced into Escherichia coli, only the recombinant truncated GR2 is soluble, expression markedly improved by co-expression of the GroES/GroEL chaperone |
| 1.1.1.79 | expressed in Escherichia coli |
| 1.1.1.79 | expressed in Escherichia coli BL-21(DE3) Rosetta (pLysS) cells and in Nicotiana tabacum BY-2 cells |
| 1.1.1.79 | expressed in Escherichia coli BL21(DE3) cells |
| 1.1.1.79 | expressed in Escherichia coli BL21-CodonPlus (DE3)-RIPL cells |
| 1.1.1.79 | expressed in Escherichia coli BL21-CodonPlus(DE3)-RIL and Escherichia coli B834(DE3)pRARE |
| 1.1.1.79 | expressed in Escherichia coli Rosetta (DE3) cells |
