EC Number |
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1.1.1.42 | 2 polymorphic isozymes IDHP-A and IDHP-B, isozymes show allelic variants in populations belonging to different living heights in the stream, analysis of allelic frequencies, overview |
1.1.1.42 | construction of a genomic cDNA library, gene Cl-idh, DNA sequence determination and analysis, expression in Escherichia coli strain BL21(DE3) |
1.1.1.42 | DNA and amino acid sequence analysis |
1.1.1.42 | DNA and amino acid sequence determination and analysis, expression as Strep-tagged protein in Escherichia coli BLR(DE3) exclusively in inclusion bodies, removal of 27 amino acids from the N-terminus results in expression of partially soluble protein |
1.1.1.42 | exchange of promotors and altering of organellar targeting for expression of isozymes IDO2 and IDO3 in mitochondria, and of isozymes IDP1 and IDP3 in the cytosol, functional complementation studies using mutant strains |
1.1.1.42 | expressed in enzyme-defective Escherichia coli strain DEK2004 |
1.1.1.42 | expressed in Escherichia coli |
1.1.1.42 | expressed in Escherichia coli BL21 |
1.1.1.42 | expressed in Escherichia coli BL21 cells |
1.1.1.42 | expressed in Escherichia coli BL21 Gold (DE3) cells |