EC Number   |
|---|
   1.1.1.103 | - |
| 1.1.1.103 | cloned as a 3562-base pair EcoRI fragment |
| 1.1.1.103 | DNA and amino acid seuence determination and analysis, sequence comparisons, phylogenetic tree, expression of His-tagged enzyme in Escherichia coli strains JM109 and BL21(DE3) |
| 1.1.1.103 | expressed in Escherichia coli BL21(DE3) cells |
| 1.1.1.103 | expression in Escherichia coli |
| 1.1.1.103 | expression in Escherichia coli strain BL21(DE3) |
| 1.1.1.103 | gene tdh, functional expression in Escherichia coli |
| 1.1.1.103 | into pET-30a digested with KpnIEcoRI to construct pET-ste11 and expressed in Escherichia coli BL21 |
| 1.1.1.103 | ligated into the pTZ57R/T cloning vector. The recombinant pTZ-tdh plasmid digested with NcoI and EcoRI and the TDH gene ligated with pET-8c, giving the recombinant pET-tdh plasmid, used to transform Escherichia coli DH5alpha competent cells. Recombinant and purified pET-tdh plasmid expressed in Escherichia coli BL21 (DE3) |
| 1.1.1.103 | orf PH0655, amino acid sequence determination and analysis, expression of His-tagged enzyme in Escherichia coli strain TOP10 |
