Refine search

Search Cloned(Commentary)

show results
Don't show organism specific information (fast!)
Search organism in taxonomic tree (slow, choose "exact" as search mode, e.g. "mammalia" for rat,human,monkey,...)
(Not possible to combine with the first option)
Refine your search

Search term:

Results 1 - 5 of 5
EC Number
Commentary
Reference
expressed in lanosterol synthase deficient (erg7) Saccharomyces cerevisiae strain GIL77
gene CBS, DNA and amino acid sequence determination and analysis, digital gene expression (DGE) profile analysis, sequence comparisons and phylogenetic analysis of codon-optimized gene, SgCbQ is functionally expressed in the lanosterol synthase-deficient Saccharomyces cerevisiae strain GIL77 leading to accumulation of cucurbitadienol as the sole product
gene CBS, subcloning in Escherichia coli strain DH5alpha, 11-oxo-24,25-epoxy cucurbitadienol as well as 11-oxocucurbitadienol and 11-hydroxycucurbitadienol are produced when enzyme CYP87D18 is co-expressed with Cyt P450 reductase and SgCbQ in Saccharomyces cerevisiae strain BY4741-Z35 microsomes, metabolite structures are confirmed by liquid chromatography-solid-phase extraction-nuclear magnetic resonance-mass spectrometry coupling (LC-SPE-NMR-MS). 11-Oxo-24,25-epoxycucurbitadienol might be a derivative of 24,25-epoxycucurbitadienol, which is assumed to be formed through cyclization of 2,3-(S)-22,23-(S)-dioxidosqualene in yeast cells by recombinant SgCbQ, and then sequentially oxidized by recombinant CYP87D18 at the C-11 position in BY4741-Z5 as the oxidation process of cucurbitadienol
gene CcCDS2, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, functional recombinant expression in Saccharomyces cerevisiae
gene CpCDS2, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, functional recombinant expression in Saccharomyces cerevisiae
Results 1 - 5 of 5