EC Number   |
Reference  |
|---|
    5.1.3.14 | bifunctional enzyme EC 5.1.3.14/EC 2.7.1.60, expressed in COS7 cells |
2279 |
| 5.1.3.14 | high level overexpression of the active enzyme is established by using the baculovirus/Sf9 system |
641739 |
| 5.1.3.14 | overexpression in Escherichia coli |
649142 |
| 5.1.3.14 | cloning of the neuC gene into an intein expression vector to facilitate purification |
651736 |
| 5.1.3.14 | expression in Sf9 insect cells, wild-type enzyme and mutant enzymes DELTA1-39, DELTA1-234, DELTA1-356, DELTA383-722, DELTA490-722, DELTA597-722, DELTA697-722, DELTA717-722 |
660988 |
| 5.1.3.14 | overexpression in Escherichia coli BL21(DE3), wild-type enzyme and mutant enzymes D100N, E122Q and D131N |
661102 |
| 5.1.3.14 | wild-type and mutant enzymes expressed in Escherichia coli |
661247 |
| 5.1.3.14 | expression of M712T mutant enzyme in Sf9 insect cells |
661735 |
| 5.1.3.14 | expression of wild-type and mutant enzymes (C13S, H132Q, D176V, D177C, V331A, I472T, G708S, A631V, V572L and A630T) in COS-7 cells |
662186 |
| 5.1.3.14 | functional expression in Escherichia coli, Saccharomyces cerevisiae, Pichia pastoris or insect cells. In all cell types, the expressed enzyme displayes both epimerase and kinase activities. In Escherichia coli up to 2 mg protein/l cell culture is expressed in yeast cells only 0.4 mg/ml, in insect cells up to 100 mg/L. In all three cell systems, insoluble protein aggregates are also observed. Expression and purification of the UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase in Sf-900 cells can yield the miligram amount of protein required for structural characterization of the enzyme. The easier expression in Escherichia coli and yeast provides sufficient quantities for enzymatic and kinetic characterization |
663299 |
