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EC Number
Commentary
Reference
a 339 bp core fragment and full-length DNA of MECPS amplified and subcloned into pGEM T-easy vector. Escherichia coli harboring both of the plasmids, pAC-BETA and pTrc or pTrcTmMECPS or pTrcAtMECPS
amplification product digested with BamHI and PstI and ligated into plasmid pQE30, expressed in Escherichia coli strain XL1-Blue
cloned into pET17b for expression of the native polypeptide in Escherichia coli strain BL21
expressed in Escherichia coli
expressed in Escherichia coli strain BL21 (DE3)
expressed in Escherichia coli strain EB370
expression in Escherichia coli
expression in Escherichia coli strain NMW26 and Arabidopsis thaliana
final amplification product digested with the restriction endonucleases BamHI and SalI and cloned into plasmid pQE30, expressed in Escherichia coli strain XL1-Blue
Results 1 - 10 of 10