EC Number   |
|---|
  4.2.3.44 | DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression of wild-type and mutant enzymes in Saccharomyces cerevisiae strain YS100, subcloning in Escherichia coli strain TOP10, transient heterologous expression of wild-type and mutant enzymes in Nicotiana benthaminana via transfection with Agrobacterium tumefaciens strain LBA4404, quantitative RT-PCR enzyme expression analysis |
| 4.2.3.44 | expression in Escherichia coli |
| 4.2.3.44 | functional expression of Picea abies isopimara-7,15-diene synthase (diTPS-ISO) in Nicotiana benthamiana leaves via Agrobacterium tumefaciens transfection method, coexpression with a multifunctional cytochrome P450 (CYP720B4) from Picea sitchensis, subcloning in Escherichia coli strain NEB10beta |
| 4.2.3.44 | gene KSL6, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged codon-optimized KSL6 in Escherichia coli strain Tuner(DE3) |
| 4.2.3.44 | transient heterologous expression of the enzyme in Nicotiana benthaminana via transfection with Agrobacterium strain GV3101 quantitative real-time PCR enzyme expression analysis |
