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expression in Escherichia coli
the modified citramalate synthase gene cimA from Methanococcus jannaschii is expressed in Escherichia coli. Gene knockouts and fermentation optimization improve citramalate production from glycerol and also reduce acetate accumulation. Near elimination of acetate formation necessitates deletions in genes for both pathways associated with acetate formation: ackA coding acetate kinase, pta coding phosphotransacetylase, and poxB coding pyruvate oxidase
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