EC Number   |
Reference  |
|---|
    3.5.1.6 | cDNA encoding human beta-ureidopropionase gene, BUP-1 cloned, plasmid yc28d10.r1 sequenced and expressed in Escherichia coli BL21, eukaryotic expression vector pCR3 transfected to COS-7-cells |
172069 |
| 3.5.1.6 | expression in Escherichia coli B843(DE3) |
654071 |
| 3.5.1.6 | cDNA truncated at the N-terminus by 14 residues, overexpression in Escherichia coli BL21 |
657030 |
| 3.5.1.6 | mutation A85E is introduced into the wild-type UPB1 plasmid using site-directed mutagenesis. The mutated plasmid is then transfected into the RKO cell line |
689360 |
| 3.5.1.6 | - |
695782 |
| 3.5.1.6 | expression of GFP-tagged PYD3 in Arabdiopsis thaliana via Agrobacterium-mediated transformation using the floral dip method. Quantitative real-time RT-PCR analysis of PYD expression in wild-type and pyd mutants, overview |
700487 |
| 3.5.1.6 | gene UPB1, genotyping |
712679 |
| 3.5.1.6 | hexahistidine fusion protein expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL |
719454 |
| 3.5.1.6 | His-tagged version expressed in Escherichia coli |
720104 |
| 3.5.1.6 | gene UPB1, located on chromosome 22q11.2, DNA and amino acid sequence determination and analysis, sequence comparisons and genomic organization analysis, genotyping of beta-ureidopropionase deficient patients as well as the analysis of the mutations in a three-dimensional framework, PCR enzyme expression analysis, overview. Recombinant heterologous expression of wild-type and mutant enzymes in Escherichia coli strain C41(DE3)pRARE2, all mutations yield mutant beta-ureidopropionase proteins with significantly decreased activity |
733382 |
