EC Number |
Reference |
---|
3.5.1.13 | - |
697867 |
3.5.1.13 | cloned in a modified pET21 vector encoding a 6x-histidine tag at the carboxyl terminus and overexpressed in Escherichia coli BL21 (DE3) |
752657 |
3.5.1.13 | DNA and amino acid sequence determination and analysis, phylogenetic analysis, genetic library construction and cloning in Escherichia coli strains NM522 and DH5alpha, expression in Escherichia coli strain BL21(DE3) |
713035 |
3.5.1.13 | gene azl13, DNA and amino acid sequence determination and analysis, sequence comparison, recombinant expression of His6-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) |
733190 |
3.5.1.13 | gene pamH, DNA and amino acid sequence determination and analysis, sequence comparison, cloning and expression of His6-tagged PamH in Escherichia coli strains DH5alpha and BL21 (DE3), respectively |
718672 |
3.5.1.13 | gene pamh, genetic organization, DNA and amino acid sequence determination and analysis, sequence comparison, recombinant expression of His6-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3). The enzyme is only successfully expressed using autoinduction media under low-temperature incubation at 25°C, otherwise, the expressed proteins forms inclusion bodies |
718672 |