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Results 1 - 10 of 10
EC Number
Cloned (Commentary)
BLLJ_1840, phylogenetic tree, expresion as soluble enzyme in Escherichia coli
DNA and amino acid sequence determination and analysis, domain structure, phylogenetic analysis, recombinant expression of C-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3)
DNA and amino acid sequence determination and analysis, expression of the gene encoding the enzyme fused to a yeast secretion signal sequence under control of the alcohol oxidase promoter in Pichia pastoris
expressed in Escherichia coli
gene BBNG_01788, sequence comparisons, recombinant expression of Trx-His-tagged enzyme in Escherichia coli strain Rosetta (DE3)
gene Fo1,3 gal, recombinant expression in Pichia pastoris strain KM71H, subcloning in Escherichia coli strain DH5alpha. The expression vector pPICZ?A contains the native Saccharomyces cerevisiae alpha-factor secretion signal, which allows Pichia pastoris to secrete heterologous proteins into the growth medium
Pc1,3Gal43A, expression in Pichia pastoris as selenmethionine-labeled protein
recombinant expression of N-terminally His-tagged wild-type and selenomethionine-labeled enzymes in Escherichia coli strain BL21(DE3)
SGalase1, DNA and amino acid equence determination and analysis, sequence comparison, phylogenetic analysis and tree; SGalase2, DNA and amino acid equence determination and analysis, sequence comparison, phylogenetic analysis and tree
Results 1 - 10 of 10