EC Number |
Reference |
---|
3.2.1.123 | - |
646701, 749565 |
3.2.1.123 | amplified gene product encoding amino acid residues 23-488 without the signal peptide digested with HindIII and NotI and inserted into the corresponding sites of pET22b(+) to generate a C-terminal His6-tagged protein. The recombinant plasmid and mutants expressed in Escherichia coli strain BL21(DE3) |
680753 |
3.2.1.123 | EGCase and EGCase P |
646695 |
3.2.1.123 | EGCase II |
646696 |
3.2.1.123 | expressed in Escherichia coli BL21(DE3) cells |
703903, 732178 |
3.2.1.123 | expressed in Rhodococcus erythropolis JCM3201 cells |
732295 |
3.2.1.123 | expression in Chinese hamster ovary cells and Escherichia coli |
665547 |
3.2.1.123 | expression in Escherichia coli BL21 (DE3) |
751102 |
3.2.1.123 | mutant E351S in pET28a subcloned into pTKNd and pUC18 at the NdeI/SalI and EcoRI/SalI restriction sites, respectively. EGC and its mutants expressed in Escherichia coli BL21 (Tuner) cells |
695545 |
3.2.1.123 | recombinant EGC, lacking the 30 amino acid N-terminal signal sequence overexpressed in Escherichia coli |
677557 |