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EC Number
Commentary
Reference
amplified gene product encoding amino acid residues 23-488 without the signal peptide digested with HindIII and NotI and inserted into the corresponding sites of pET22b(+) to generate a C-terminal His6-tagged protein. The recombinant plasmid and mutants expressed in Escherichia coli strain BL21(DE3)
EGCase and EGCase P
EGCase II
expressed in Escherichia coli BL21(DE3) cells
expressed in Rhodococcus erythropolis JCM3201 cells
expression in Chinese hamster ovary cells and Escherichia coli
expression in Escherichia coli BL21 (DE3)
mutant E351S in pET28a subcloned into pTKNd and pUC18 at the NdeI/SalI and EcoRI/SalI restriction sites, respectively. EGC and its mutants expressed in Escherichia coli BL21 (Tuner) cells
recombinant EGC, lacking the 30 amino acid N-terminal signal sequence overexpressed in Escherichia coli
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